ABSTRACT
O objetivo deste estudo foi analisar a influencia do banho 24 horas antes do procedimento cirúrgico e da tricotomia pré-operatória na redução da contagem microbiana da pele do campo operatório, bem como a eficiência antisséptica do gluconato de clorexidina 0,5% e da polivinilpirrolidona iodada 10% para antissepsia do sítio cirúrgico, verificando sua ação 4 minutos e 2 horas após aplicação. Utilizaram-se 20 cadelas hígidas, alocadas ao acaso em 2 grupos de 10 animais: os animais do Grupo I foram submetidos ao banho 24 horas antes do procedimento cirúrgico, enquanto os do Grupo II não passaram por este procedimento. Foram isolados diversos gêneros bacterianos antes e após o uso dos antissépticos. Significância estatística foi verificada entre os grupos quanto ao efeito do banho prévio à cirurgia, apenas 2 horas após a aplicação de clorexidina. A tricotomia reduziu 26,48% da carga microbiana nos animais do Grupo I e elevou a carga microbiana em 41,19% nos animais do Grupo II, revelando diferença estatística. Após o uso dos antissépticos, não foi observada diferença estatística entre os grupos em nenhum momento. Do mesmo modo, a comparação da eficiência dos antissépticos com ou sem banho, não revelou significância estatística. A polivinilpirrolidona iodada causou reação alérgica em 15% dos animais e não foi observada irritação cutânea causada pela clorexidina. Conclui-se que o banho prévio tem efeito na redução da carga bacteriana apenas após 2 horas de antissepsia com clorexidina; a tricotomia é mais eficaz na redução microbiana quando o animal é submetido ao banho e os dois antissépticos são igualmente eficazes na antissepsia cirúrgica por até 2 horas quer o animal tome banho ou não previamente à cirurgia.(AU)
The objective of this study was to analyze the influence of bath 24 hours before surgery and preoperative shaving in reducing microbial count of the operating field skin and antiseptic efficiency of 0.5% chlorhexidine gluconate and 10% iodine polyvinylpyrrolidone for antisepsis of the surgical site, by checking its action 4 minutes and 2 hours after application. We used 20 healthy bitches, randomly allocated into 2 groups of 10 animals: Group I underwent bath 24 hours before surgery, whereas Group II did not undergo this procedure. Many bacterial genera have been isolated before and after use of antiseptics. Statistical significance was observed between the groups regarding the effect of bath prior to surgery, just two hours after application of chlorhexidine. Shaving reduced 26.48% of the microbial load in Group I and increased the microbial load in 41.19% in Group II, showing statistical difference. After the use of antiseptics, there was no statistical difference between the groups at any time. Similarly, comparison of the efficiency of the bath with or without antiseptic showed no statistical significance. The iodine polyvinylpyrrolidone caused allergic reaction in 15% of animals and was not observed skin irritation caused by chlorhexidine. We conclude that the prior bath is effective in reducing bacterial load just after 2 hours of antisepsis with chlorhexidine; shaving is more effective in reducing microbial when the animal is subjected to the bath and the two antiseptic agents are equally effective in surgical antisepsis or for up to 2 hours the animal did not take a bath or prior to surgery.(AU)
Subject(s)
Animals , Female , Dogs , Antisepsis , Dogs/surgery , Hair Removal/adverse effectsABSTRACT
OBJECTIVE: To prepare PVP-I film agent of high water solubility and to establish a method for its quality control. METHODS: The film was prepared by mixing PVP-I,glycerol, distilled water and PVA05-88. The content of povidone iodine was determined with electric titration. The stability of the preparation stored in brown glass and colorless glass was investigated.RESULTS:The prepared film was integrated, clear and even in color and size. The average recovery was 99.03%(RSD=0.75%).The film stored in brown glass showed no evident changes in appearance and content within 60d, while that in colorless glass was found to have a decrease in content and yellowish appearance. CONCLUSION: The preparation technology is simple and method of quality control is accurate and reliable.
ABSTRACT
OBJECTIVE:To prepare anhydrous iodine-containing or iodine-free hand-cleaning agents for use at field or water-deficient area.METHODS:An adhesive paste was made with PVA as vehicle and PVP-I as disinfector.Iodine value was taken as parameter of quality control.The adhesive-eliminating test was carried out on Escherichia coli and HBV elimi?nating and skin irritation tests were performed in rabbits.RESULTS:This preparation could eliminate bacteria by its adhesive action and had considerable disinfective action because of the ingredient iodine,however it could not eliminate HBV.No irrita?tion to skin was found.CONCLUSION:The preparation can effectively remove the dust,bacteria and viruses from the hands and is suitable for cleaning hands in conditions of wartime,training and water-deficient area.